Nova Male Fertility Test (2)

Nova Male Fertility Test is an antigen-capture immunochromatographic assay, detecting sperm antigen in semen samples. Monoclonal antibodies specifically against sperm antigen are 1) conjugated with colloidal gold and deposited on the conjugate pad, and 2) immobilized on the test line of the nitrocellulose membrane. When sample is added, the gold-antibody conjugate is rehydrated and sperm antigens interact with the gold conjugated antibodies. The antigen-antibody-gold complex then migrates towards the test window until the Test Zone (T) where it will be captured by immobilized antibodies,forming a visible pink line (Test band), indicating a positive result. If sperm concentration is below normal limit (15 million/ml) in the sample, no pink line will appear in the Test Zone (T), indicating a negative result. To serve as an internal process control, a control line should always appear at Control Zone (C) after the test is completed. Absence of a colored control line in the Control Zone is an indication of an invalid result.